Seahorse XF Analysis Platform
Live-cell, functional metabolic data is impacting therapeutic research and discovery in areas like these:
Immunology
- Determining metabolic fitness of immune cells
- Measuring and modulating immune cell activation in real time
- Targeting metabolic pathways to improve immunotherapy design and performance
Cancer
- Rewiring metabolism to support rapid growth
- Understanding which nutrients fuel cancer cells
- Uncovering how metabolism impacts the tumor microenvironment
Toxicology
- Detecting mitochondrial liabilities among potential therapeutics
- Assessing the risk of mitochondrial toxicity in vitro with high specificity and sensitivity
- Determining the mechanism of action of mitochondrial toxicants
Obesity, diabetes, and metabolic disorders
- Measuring functional effects of genetic changes to metabolic pathway components
- Examining nutrient use in healthy and diseased cell models
- Assessing fatty acid oxidation and glycolysis in different cell types
The standard for measuring energy metabolism in live cells
Agilent Seahorse XF analyzer revolutionized the measurement of bioenergetics in live cells in real time. Seahorse XF analyzers enable robust measurements of mitochondrial activity, glycolysis, and ATP production rates in a microplate format.
- Automatic calculation of oxygen consumption and proton efflux rates
- Label-free detection of discrete bioenergetic changes in live cells—in real time
- Compatibility with both adherent and suspension cells, as well as isolated mitochondria
- Instrument-controlled gentle mixing, measuring, and compound injections
- High sensitivity to analyze as few as 5,000 cells per well
- Temperature controlled to maintain cell health and kinetics
